Allosteric Properties of Skeletal Muscle Pyruvate Kinase
نویسندگان
چکیده
منابع مشابه
Allosteric properties of skeletal muscle pyruvate kinase.
The phenylalanine inhibition of skeletal muscle pyruvate kinase has been further studied. Even at high levels of the inhibitor the plots of reaction rates as a function of phosphoenolpyruvate concentration give hyperbolic curves; the inhibition by phenylalanine is of the mixed type. The enzyme exhibits a homotropic cooperative effect with respect to this inhibitor. This property is strongly dep...
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Hitherto it has been generally supposed that pyruvate kinase (EC 2.7.1.40) has no significant role in the regulation in vivo of glycolytic flux in skeletal muscle (Carbonell et al., 1973). This supposition, apart from providing an explanation for the apparent lack of potentially regulatory allosteric interactions by skeletal-muscle (M-type) pyruvate kinase, is in agreement with the idea that gl...
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Conversion of lactate to glucose was examined in myotubes, minced muscle tissue, and rats exposed to 2H2O or 13C-enriched substrates. Myotubes or minced skeletal muscle incubated with [U-(13)C3]lactate released small amounts of [1,2,3-(13)C3]- or [4,5,6-(13)C3]glucose. This labeling pattern is consistent with direct transfer from lactate to glucose without randomization in the tricarboxylic aci...
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Erythrocyte pyruvate kinase (PK) is an important glycolytic enzyme, and manipulation of its regulatory behavior by allosteric modifiers is of interest for medicinal purposes. Human-erythrocyte PK was expressed in Rosetta cells and purified on an Ni-NTA column. A search of the small-molecules database of the National Cancer Institute (NCI), using the UNITY software, led to the identification of ...
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The enzyme from most sources is a tetramer of identical subunits each about 500 residues in length. The enzyme has an absolute requirement for monovalent and divalent cations (usually K+ and Mg2+) which act to coordinate and orientate the substrates prior to catalysis. A number of isoenzymes of pyruvate kinase are found to exist in vertebrate tissues. Their kinetic and regulatory properties ref...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)45884-5